Summary
Type: SPE
Application #: 300820
Catalog#: 730077 - SPE Cartridges, Chromabond, SA, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume methanol / water (1 : 1, v/v), then 1 column volume 0.1 N
phosphate buffer pH 6.5
Sample application:
Slowly force or aspirate 500 μl sample through the column
Column washing:
0.1 N phosphate buffer pH 6.5, methanol / water (1 : 1, v/v)
Elution:
Force or aspirate 2 x 250 μl OPA (o-phthalaldehyde) / mercaptoethanol reagent
through the column, let eluate stand 5 min, then elute into the same vessel
using 1 ml methanol
Authors: Macherey-Nagel
Source: LC department